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Genomics - Coggle Diagram

- - - - kolonisatie colon
    - - nieuwe niche
  - - - hogere conc.GC in DNA?
        
        nee, wel in tRNA en rRNA, ook extra gen voor ‘reverse gyrase’: relaxatie supercoiled DNA
- - - - mix single strands of DNA with primer, DNA polymerase I, 4 deoxynucleotides (one of which is radiolabeled), small amount of one ddNTP
      - DNA synthesis occurs; random insertion of ddNTP generates DNA fragments of different lengths
      - four reactions carried out; each with different ddNTP
      - fragments in each reaction mixture separated electrophoretically
      - gel autoradiographed and sequence read
  - - - analysis of genome data using computers
      - generates data on genome content, structure, and arrangement
      - also provides data on protein structure and function
      - uses annotation to determine location of genes on newly sequenced genome
      - further examination carried out using in silico analysis
  - - - a reading frame < 100 codons that is not interrupted by a stop codon
      - there is an apparent ribosomal binding site at the 5’ end and terminator sequences at the 3’ end
- - - - motif, a short pattern of amino acids, may represent a functional unit within the protein,such as the active site
        :!:
  - - - targets are labeled with fluorescent dyes and then incubated with the gene chip
      - unbound target is washed off
      - the chip is scanned with laser beams to detect fluorescence which indicates that hybridization has occurred