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Experiment 1, Experiment 2, Nanodrop, In Karatsu, Artificial reproductive,…
Experiment 1
Adult Chub Mackerel
Seedlings
Collect sample for large and small groups
DNA extraction
Pooled seq library preparation (12 samples/groups)
Next generation sequencing
SNP analysis
RNA extraction
Measure gene expression
Experiment 2
Collect sample of 5-11 months of farm-raised Chub Mackerel
Collect samples for male and female groups
Nanodrop
In Karatsu
Artificial reproductive
Measure fork length
MagBead protocol
Caudal fin
Gonad
RT-PCR
DNB seq platfom
CLC genomic workbench
In Matsumoto Suisan and Ao Suisan