Please enable JavaScript.
Coggle requires JavaScript to display documents.
20BT2068- PRINCIPLES OF PLANT BIOTECHNOLOGY AND APPLICATIONS - Coggle…
20BT2068- PRINCIPLES OF PLANT BIOTECHNOLOGY AND APPLICATIONS
Module 1: Plant Tissue Culture
Organogenesis–direct and indirect (meristem/shoot apex culture, callus and suspension culture)
Significance and application of anther culture, ovule culture, embryo culture
Types of media and their preparation plant hormones
Somatic embryogenesis, protoplast fusion
History-tissue culture lab - establishing aseptic conditions
Somaclonal variation-artificial seeds
Micropropagation
Hardening and acclimatization of tissue cultured plants
Module 2Plant Genetic Engineering Tools
Chloroplast Transformation
Protoplast transformation
Biolistic,PEG/liposome-mediated, electroporation
Plant transformation methods-stable and transient - Agrobacterium-mediated
Site directed integration of transgene (zinc finger).
Biology of Agrobacterium tumefaciens
Module 4: Secondary metabolite production in tissue culture
Hairy root culture
Immobilization, permeabilization
Biotransformation, elicitation
Callus culture initiation
Module 5: Plant Breeding and Protection
Marker assisted selection
cultivar release and commercial seed production
Genetic resources for breeding, Germplasm conservation
Biotic stress factors and natural disease resistance pathways
Sexual hybridization Mutagenesis – Polyploidy
Abiotic stress factors - tolerance mechanisms
Module 3: Vectors in plant transformation
Binary and co-integrate vectors
Gateway vectors-promoters-selectable and screenable markers
legume symbiosis, N2 Fixation; Regulation of NIF and NOD Genes
Marker free transgenics-significance and applications
Crop improvement
Plant as Bioreactors- edible Vaccines
Gene Banks;
Germplasm conservation
Module 6: Bioreactors for drug production
Types of bioreactors for plant cell suspension culture
Re- and multi usable bioreactors for plant cell suspension culture
Bioreactors: In-Process control (IPC), determination of plant cell growth: Illumination
Single-use and disposable bioreactors for plant cells and tissue cultures
Re- and multi usable bioreactors for root culture
Single use vs re- and multiusable bioreactors. Advantages and disadvantages