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Lab Pod Practical Skills Assessment - Coggle Diagram
Lab Pod Practical Skills Assessment
Microscope analysis
Passage number written with "P" at the beginning
Best confluency to treat cells at is 40%
Protein
Calculating protein volume
Convert ug/mL to ug/uL
Divide total required by concentration
For 4X sample buffer divide desired total volume of mixture by 4.
For 10X sample buffer divide desired total volume of mixture by 10.
Make up remaining volume with water
Remaining lysates should be stored at -20C
Antibodies
Look at protein name and cell line.
Choosing primary antibody
Make sure it has a reactivity to the species of the sample you are testing (e.g. human)
Make sure it has an application in the procedure you are using (e.g. WB = western blot)
Appropriate dilution ratio for procedures found on information sheet.
Secondary antibody species should match the species that the primary antibody came from.
Visualising bands using iBright: use Chemi blots.
Labelling tubes (e.g. 0.4M Tris Buffer)
NAME
CID
CONCENTRATION AND REAGENT
DATE
Put these on a sticker on the container (not the lid)