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DNA replication and repair - Coggle Diagram
DNA replication and repair
replication
semiconservative
proved by Meselon-Stahl experiment
bidirectionally
proved by Carin's experiment
semidiscontinuous
one leading strand and one lagging strand
replication always start at a unique origin
shown in Inman'sexperiment
always goes from 5' to 3'
enzymes used in DNA synthesis
DNA pol I
primarily used for clean up
DNA pol II IV and V
involved in repair
DNA pol III
replication polymerase
DNA ligase
seal nicks between nucleotides
topoisomerase
relieve stress of underwinding
termination
two replication forks meet at Ter site
causing replication fork to stop
lesioin= DNA damage
if unrepaired lesion becomes mutation
types of DNA damage
mismatch
rely on methylation
abnormal bases
use DNA glycosylases
pyrimidines forms dimers
repair with photolyase or ABC excinuclease
backbone lesion
repair with NHEJ or HR