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Arg-sensor (2023_Q1) - Coggle Diagram
Arg-sensor (2023_Q1)
NIR-Arg sensor
ArtJ_entry135
with linear BphPs
test
exp/ puri
cloning
with cpBphPs
Mammalian Expression
Hela Cell testing
WB
Fluo. Images
Mamm. plasmid cloning
rsGreenAr_entry135
Photoswitchable version
Clone rsEGP2 muts
exp & puri
test
Entry site refinement
Linker targeted-mutants
Site-specific mutagenesis cloning
Candidate validation
Expression / Purification
Testing (spectra; titration)
Linker library
Library cloning
Library high-throuput screening
Candidate validation
DNA isolation
Expression/Purification
Testing (spectra; titration)
High-throughput screening
Bacterial Arg uptake assay
Search lit
Standard curve with GrAr11
Troubleshoot NIR-setup