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Transcription Factor, hypothese to explain the ability of enhancers to act…
Transcription Factor
Gene-specific transcription factor
Activitor
bind to enhancer
provide extra needed boost to transcription
stimulate or inhibit transcription by RNA pol ll
structure
DNA-binding domain (DBD)
homeodomains
60 amino acids
helix-turn-helix
homeobox proteins
helix in major groove
N-terminal in minor groove
bZIP and bHLH
highly basic DNA-binding motif linked to protein dimerization motifs
leucine zippers
bZIP
Helix-loop-helix
bHLH
(transactivitor complex) Myc-Max
zinc-containing modules
all use one or more zinc ions to create a shape to fit an alfa helix of motif into the DNA major groove
Zinc modules
Glucoticoid Receptors(GR)
protein-protein interaction to form dimers
Nuclear Receptors(NR)
Type ll
stay in nucleus bound to target DNA site (THR-RXR)
active without or with ligands(9-cic RA)
Type lll
No ligands identify
Type l
find in cytoplasm bound to another protein (GR)
bind to hormone ligands
relase the cytoplasmic protein (Hsp90)
1 more item...
endocrine-signaling
protein+endocrine molecule = complex bind to hormone response element = [Activitor]
stimulating transcription of associated genes
(GAL4)
2 zinc ions and 6 cysteines (bimetal tiolate cluster)
No zinc fingers
Short alfa helix (4zinc) in DNA major groove (recognition module)
Dimeization motif with alfa helix
colied coil
Zinc fingers (TFlllA, Sp1)
antiparallel beta sheet (2cys)
coordinated to a zinc ion
alfa helix (2His)
12 aa
motifs
Transcription-activition domain (TAD)
one or more domains of
Glutamine-rich domains
Proline-rich domains
Acidic domains
dimerization domain
increase the affinity between activitor and target DNA
Activitor forms
homodimers
hetrodimers
General transcription factor
hypothese to explain the ability of enhancers to act at a distance
looping
detact by Chromosome conformation capture (3C)
change in topology
supper colied
sliding
facilitated tracking
independent modules
recruitment of preinitiation complex component
RNA polymerase holoenzyme
GTFs already to polymeras
stepwise build-up
come one by one to bind
more correct
suzan albordaini
201703178