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Eukaryotic RNA polymerase incaple to bind by himself to promoter, NC2,…

- - - - TFllB
        
        bind next (DAB complex)
        
        bind to polymerase ll , NTD
        
        bint to TBP, CTD
        
        establishing the transciption start site (active center)
      - TFllA
        
        Help D to be required
      - TFllH
        
        9 subunits
        
        protein kinease complex (4 subunits)
        
        Phosphorylates the CTD of RNA pol ll in serine 5
        
        ll a to ll o
        
        esdential for initiation
        
        core TFllH complex (5 subunits) with 2 DNA helicase/ATPase activites
        
        Unwinds DNA downstream at the transcription start site to create the transcription bubble
        
        RNA pol ll leave the promoter
        
        last factor bind to preinitiation complex (DABPolFEH complex)
      - TFllD
        
        bind to TATA box with help of TFllA to form (DA complex)
        
        contains several subunits
        
        13 core TBP-associated factors (TAFs)
        
        functions
        
        interaction with the core promoter elements
        
        intrraction with gene-specific transcription factors
        
        when attached to TPB extend the binding of TFllD beyond TATA box
        
        roles of TAF1 and TAF2
        
        help TFllD bind to initiator and DPE of promoter
        
        enable TPB to bind to TATA-less promoter (GC box)
        
        TAF 1 enzymatic activities
        
        Hisrone acetyltransferase
        
        remove the colid in histone of DNA
        
        Prorein kinase
        
        phosphorilation
        
        TATA-box binding protein (TBP)
        
        Highly evolutionarily conserved (80% in aa)
        
        Bind to minor groove of TATA box
        
        80 degrees curve
        
        universal transcription factor in all three classes
        
        alternative protein
        
        TRF1 (TBP-related factor)
        
        TLF (TBP-like factor)
        
        interaction between TBP and Promoter
        
        TATA-less promoter with Initiator and DPE
        
        TAFs 1,2 bind to Inr first to help TPB to bind
        
        TATA-less promoter with GC boxes
        
        Sp 1 bind to GC
        TAF4 bind to sp1
        help others bind to sp1 TAFs 1,2 and TBP
        
        TATA-containg promoter
        
        TPB bind directly to TATA than TAFs 1,2 bind and others
        
        TFTC
        
        not have TBP , but some TAFs
        
        5 Domain
        
        similar 3D structure of TFIID
      - TFllF
        
        required/ helps RNA polymerase bind to a region
        from -17 to +17 (DABPolF)
      - TFllE
        
        Requiered TFllH
        
        help and stuimelate TFllH to phosphorylate RNA Pol ll a
        
        required for promoter clearance
        
        between initiation and elongiation
        
        allow DNA melting / helicase activity in TFllH
      - in addition to
        
        RNA polymerase :
        
        Gives
        
        preinitiation complex
      - Mediator complex
        
        not required for initiation but it is required for activated transcription
        
        without
        
        basel transcription
      - dictate the starting point and direction
    - - Activitor/Repressor
- - - - DRB sensitivity inducing factor
    - - negative elongiation factor
  - - - positive elongiation factor