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Cut&tag sequencing - Coggle Diagram
Cut&tag sequencing
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general info
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fresh is best as fixing can destroy histones, but light fixation same as for HiC is okay
100,000 cells per library (as little as 500 but not as good as 2 copies per gene so more is better)
if 'light fix' before sort then can store cells after sort at 4oC for 1-2weeks, after this in sodium azide