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STAINING METHOD, RAKOFF METHOD
The Rakoff supravital method is a quick…
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RAKOFF METHOD
The Rakoff supravital method is a quick-dyeing or staining procedure for assessing hormone status. The epthelium can be separated between eosinophilia and cyanophilia. Smears of the vaginal and cervical epithelium are used.
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METHOD
- Take a specimen of material using a moistened cotton bud and apply to the specimen holder as a thin smear.
- Immediately drip one drop of Rakoff’s solution onto the smear, cover with a cover glass, and leave to react.
- Evaluate under the microscope after 1-5 minutes.
INTREPRETATION
- Cytoplasm – superficial cells found in orange or pink
- intermediate cells found in blue-green or blue-grey
- Vesicular nuclei - with distinctly stained edges, otherwise weakly stained
INTREPRETATION
- Nuclei - brown-red/Cyanophilic (basophilic)
- cytoplasm - blue-green - Eosinophilic (acidophilic)
- cytoplasms - red
SHORR'S METHOD
To prepare a gynecologic specimen, distinguish the acidophilic (eosinophilic) and basophilic (cyanophilic) cells. The quantity of follicular vs corpus luteum sex hormones was evaluated using two kinds of cells. The stain for acidophilic cells is red, whereas the stain for basophilic cells is blue-green (cyan).
METHOD
- Place in distilled water for 2 min
- Place in alcohol at 95° for 2 min
- Rinse in running water for 2 min
- Place in the solution of Q Path Harris’s haematoxylin for 2 min
- Rinse in running water for 2 min
- Place in alcohol at 70° for 2 min
- Place in alcohol at 95° for 2 min
- Place in the solution of Q Path Shorr for 2 min
- Place in alcohol at 95° for 2 min
- Place in alcohol at 100° for 2 min
- Place in xylene for 2 min
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- Assemble the slides using the Q Path Coverquick range
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GROUP MEMBERS
- ABIGAIL PRIYANKA THOMAS (012020090673)
- ROSHINI A/P ASOGAN (012020091620)
- SANGERRTENA KUNASEKARAN (012020092370)