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Welcome to MSCK 1213 Flipped Classroom, where students are the '…

- - - - acrylamide-based :
      - methacrylate-based
      - styrene‑based
  - - - Advantages
        
        work at high flow rate without generating high back pressure
        
        stable over wide pH range
        
        high efficiency without the need of high pressure
- - - - Disadvantages
        
        comparioson btw categories
        
        Advantages of High flow rates: Due to high porosity and Low backpressure: Less stress on system and column
      - Can as well minimize back pressure and enhance the flow rate of mobile phase
      - Has high surface area
      - It necessitate the use of nonpolar mobile phases when they are used directly for liquid chromatography; alternatively, the silica skeleton is subjected to a derivatization procedure to convert the monolith to a reversed phase.
      - Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and variable through-pore size/skeleton size ratios
      - The internal structure of the monolithic column is created in such a way that many channels form inside the column
      - The internal structure of the monolithic column is created in such a way that many channels form inside the column
    - - The monolithic stationary phase can be produced via polymerization reaction that yields void
- - - - contribute to high flow rate and even distribution
        
        increase the speed
        
        will reduce the back pressure
      - high surface area
        
        higher absorption capacity column
      - network of channel have no dead ends and small pores
        
        allow larger molecule to trap into the column
        
        support efficient recovery
    - - give high binding capacity for large target molecule
        
        enables fast separation of large molecule over short flow pass
        
        gives high resolution
  - - - applicable in investigation of various sample
    - - suitable for separation of large molecule such as protein and polynucleotides