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'seq project' - Coggle Diagram
'seq project'
Hogan lab
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show differentiation, that it is progressive, details maybe due to technology but same trend
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zygotic prox1a&b at 4dpf, and 48hpf for maternal zygotic showing in a and not b not so strong at 48hpf. show contribution early on via this technique - concluded prox acts as a repressor of VEC fate, very little that are activators
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datasets
have
sc RNA seq - 36hpf, 48hpf, 5dpf, 7dpf
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proxrfp + nfli GFP = ATAC, HiC, cut and tag (only lymphatics then single pos for nfli = all other EC)
paper ideas
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figures
- global and local changes in chromatin architecture
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- differentiation of LEC identity genes
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RNA scope validation
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choose 20 genes, 10 that are dynamic and high, 10 of interest - look at RNA scope see what comes out good for validation
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