Enzymes
Characteristics of Enzymes
- Heat labile
- Water-soluble
Classification
5.Isomerases
6.Ligases
4.Lyases
1.Oxidoreductases
3.Hydrolase
2.Transferases
catalyze the transportation of a functional group from one molecule to another. Prime examples include aminotransferases, which catalyze amino acid degradation by removing amino groups.
enzymes catalyze hydrolysis, where single bonds are broken down upon exposure to water. For example, glucose-6-phosphatase is a hydrolase that removes the phosphate group from glucose-6-phosphate, leaving glucose and H3PO4 (phosphoric acid).
Functions of Enzymes
catalyze the breakdown of various chemical bonds by means other than hydrolysis and oxidation, often forming new double bonds or ring structures. Pyruvate decarboxylase is an example of a lyase that removes CO2 (carbon dioxide) from pyruvate.
catalyze structural shifts in molecules, causing changes in shape. An example: ribulose phosphate epimerase, which catalyzes the interconversion of ribulose-5-phosphate and xylulose-5-phosphate.
catalyze ligation--the combination of pairs of substrates. For example, hexokinases is a ligase that catalyzes the interconversion of glucose and ATP with glucose-6-phosphate and ADP.
- Almost all enzymes are proteins
How Enzyme Work
.
- They help in generating energy in the body. ATP synthase is the enzymes involved in the synthesis of energy.
- They function to reorganize the internal structure of the cell to regulate cellular activities.
- Enzymes perform a number of biochemical reactions, including oxidation, reduction, hydrolysis, etc. to eliminate the non-nutritive substances from the body.
- Enzymes are responsible for the movement of ions across the plasma membrane.
- Enzymes help in signal transduction. The most common enzyme used in the process includes protein kinase that catalyzes the phosphorylation of proteins.
- They break down large molecules into smaller substances that can
be : easily absorbed by the body.
catalyze oxidation reactions in which electrons travel from one molecule to another. An example: alcohol dehydrogenase, which converts alcohols to aldehydes or ketones. This enzyme makes alcohol less toxic as it breaks it down, and it also plays a key role in the fermentation process.
For example, without the presence of an appropriate enzyme, the glucose molecules and phosphate molecules in glucose-6-phosphate will remain bonded. But when you Introduce the hydrolase enzyme, the glucose and phosphate molecules separate.
- Be precipitated by protein precipitating reagents (ammonium sulfate or trichloroacetic acid)
- Contain 16% weight as nitrogen.
For chemical reactions to occur, molecules must collide under appropriate conditions that enzymes can help create.