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What conditions are most favourable for enzyme function? - Coggle Diagram
What conditions are most favourable for enzyme function?
Condition
pH level
What is the most favourable pH level
At what point does the specific enzyme denaturise
Enzyme concentration
Temperature
What temperature provides the most energy without killing the enzyme
At what point is there too little energy for the enzyme to undergo its process
How much heat is too much before it kills the enzyme
SA:V ratio of enzyme
Keeping one sample of the enzyme in clumps to see how the surface area to volume ratio affects the efficiency
Natural energy
ATP
How do sugar levels affect efficiency
Sucrose
Lactose
Frucose
Glucose
Etcetera
Function
Speed up the decomposition of certain stains to make them wash away easier
Results measured every 10 minutes for 60 minutes and results recorded
Act as a catalase for the breakdown of stains
Enzyme
Lipases (fats, oils and lipids)
Grease
Pretty transluscent and is usually handled with some degree of protection form stains e.g. aprin
Butter
Once again, not very visible and not a common stain on clothes due to aorins and the like
Lipstick
I dont believe is a ery frequent stain but when it does happen it is usually a vibrant colour so could be
Amylases (starch)
Potato
Very hard for potato to get stuck in your clothes and due to it being very solid, it is pretty easy to wipe off
Oatmeal
Pretty easy to see stain, althought not many people eat oatmeal I a sure it is still a rather prominent issue
Proteases (proteins)
Blood
Known as one of the hardest stains to remove due to the fact that it binds to cloth so easily, would be a very releant investigation and it is easy to see
Egg
Not very releant as usually when people handle eggs they are wearing some sort of aprin and it is very hard to spill raw egg on yourself
Gravy
Nice thick consistency and very easy to see, but different gravy's can hvae different ingredients that may cause it to be easier or harder to clean
Sweat
Very common, but due to it being transluscent hard to measure effectivity
Grass stains
Another classic very common stain, this would be especially useful for cricket players
Chocolate
Again, not a super common stain and different chocolates may require different methods to be cleaned most effectively.
Pectinases (Pectin)
Jellies
Again, this example is very visible but jelly is not a ery common food for the average Australian and it is hard for it to get stuck in clothes with its texture
Berries
Usually quite a noticeable colour, but have a certain firmness to them that requires a much higher level of force to stain clothes making it a less frequent occurence
Jam
Very vibrant colour and common stain but due to there being lots of different types with different textures it is not effective to test a single jam type
Processed tomato
Vibrant colour, very easy to spill when rushing around the kitchen and moist consistency that allows it to be sborbed into clothes easily
Favourable
How will this be measured?
Measuring byproducts of enzyme process
Utilising a contraption that utilises the difference in weight between the gasious byproduct and oxygen, this can then be measured to after a certain amount of time to find the efficiency
Materials consumed
Measure the amount of materials consumed that are needed for the enzyme to do its process
ATP
Measure out the material before and after the experiment to find the amount consumed and compare with other samples
Oxygen
kcat/km
This is a very hard procedure to do in a school environment and requires equipment not available to us