Please enable JavaScript.
Coggle requires JavaScript to display documents.
“What conditions are most favourable for enzyme function?” - Coggle Diagram
“What conditions are most favourable for enzyme function?”
Conditions
Temperature
Too low and collision theory states there will be a decrease in reaction
Too high and the kinetic energy will deform active site
pH
Low pH deforming active site
High pH deforming active site
Substrate concentration
A higher concentration of substrate will allow for a faster rate of reaction. However, there is a point where all active sites are filled, and enzyme activity will not increase
Enzyme concentration
Presence of inhibitors
Non-Competitive
These inhibitors can affect the active site, deactivating enzymes
Competitive
In this scenario, the conditions become the dependant variable
Water content
If the water includes any unwanted particles, they may effect the rate of reaction for the enzyme, or possibly effect the substrate, changing its specificity for the enzyme.
Enzyme
What enzyme is being used?
Protease
Amalyase
Lipase
Pectinase
What subtrate?
Starches
Proteins
Oils
Pectin
What ever substrate (stain substance) is chosen, one of these corresponding enzymes will need to be selected.
Favourable
What is the use for the enzyme?
Washing machine detergents
Dish washer detergents
Other cleaning chemicals
What is the favourable outcome for the enzyme?
Time to remove stain
Quality of removal
Function
In this practical, enzymes used to remove stains in clothes, will be assessed
To break down common susbtances which make up stains
Wine
One of these will be selected, of which a corresponding enzyme will be used to break down the stain particles
Grass
Dirt
Oils
Inks
Foods
How could the cleaning ability of an enzyme-added detergent be tested?
What Variables?
Independent Variable: The rate of reaction for substrate removed
Dependant Variable: Temperature
Controlled Variables: Water content, pH, enzyme concentration, substrate concentration, age of enzyme added, age of substrate added, dilution of enzyme-added detergent.
Measuring concentration of substrate? Or measuring concentration of by-products to determine substrate concentration?
How will the tester prevent of the reaction continuing to occur, when testing for substrate concentration?