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Fluorescence - Coggle Diagram
Fluorescence
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Depending on the nature of the excited state the luminescence can be divided into either fluorescence or phosphorescence
In excited singlet states, the electron in the excited orbital is paired (by opposite spin) to the second electron in the ground state orbital
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Phosphorescence: emission of light from triplet excited states, in which the electron in the excited orbital has the same spin orientation as the ground-state electron
Emission rates are slow (10^3-10^0 1/s) so that phosphorescence lifetimes are typically millisecond to seconds
Normally not observed in fluid solutions at room temperature since there exist many deactivation processes that compete with emission (such as non-radiative decay and quenching processes)
Jablonski diagram
The process that occur between the absorption and emission of light are usually illustrated by the Jablonski diagram
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Highly sensitive - no longer a need for the expense and difficulties of handling radioactive tracers for most biochemical measurements
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