Please enable JavaScript.
Coggle requires JavaScript to display documents.
Gene Expression - Coggle Diagram
Gene Expression
Strucrure of DNA
Nucleotide
Phosphodiesterase Backbone
Phosphate group
Deoxyribose SUgar
Nitrogenous bases
Adenine
double hydrogen bond
Pyurine (Double Ring)
Thymine
double hydrogen bond
Pyrimidines(Single Ring)
Cytosine
triple hydrogen bond
Pyrimidines(Single Ring)
Guanine
triple hydrogen bond
Pyurine (Double Ring)
Double Helix
Plasmids
Circular DNA found in bacteria outside of chromosomes
Structure of RNA
Nucleotides
Phosphodiesterase Backbone
Phosphate group
RIbose Sugar(Has an extra oxygen molecule)
Nitrogenous bases
Adenine
Pyurine (Double Ring)
double hydrogen bond
Uracil
double hydrogen bond
Pyrimidines(Single Ring)
Guainine
triple hydrogen bond
Pyurine (Double Ring)
Cytosine
triple hydrogen bond
Pyrimidines (Single Ring)
Made by using DNA
3 different types
tRNA
mRNA
rRNA
Central Dogma
Transcription
DNA is unwind ed
RNA polymerase creates the mRNA sequence using DNA
Translation
ribosome binds to start codon in RNA
tRNA carries amino acids
tRNA enters in A site
Another tRNA enters in A site and the first oen moves to the p site
Another tRNA travels to A site and the tRNA move over while transfering amino acids
Once stop codon is reached the tRNA is knocked out
Polypeptide is made and folds
1 more item...
Genetic Engineering
Restriction Enzyme
Cuts Plasmid
The enzyme ligase puts attaches the gene to the plasmid
Mix in Calcium Chloride(a salt solution)
place bacteria on ice then heat shock
Plasmids ill enter bacteria and multiply
Extract proteins
Cuts desired gene from DNA
Fixing gene sequence
Crisper
Naturally occurring gene correction in bacteria
It is very ease and plasmid genes can be fixed without cloning
Can fix wrong sequence if similar
Re clone gene
Take gene of Interest and reinsert it into plasmids
Results may be the same
It's easy to redo and cheap
Custom plasmid
Fill out order form to make plasmid from scratch
You have extreme control over what is in the plasmid
It is very expensive and take a long time
Extracting plant DNA
Use soap to disrupt cell membrane
Add salt to inactivate the protiens
Add alcohol to separate the cell and the DNA
Collect DNA