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Application of Recombinant DNA Technology in Forensic Science - Coggle…
Application of Recombinant DNA Technology in Forensic Science
Traditional Techniques
Restriction Fragment Length Polymorphism (RFLP)
Short Tandem Repeat analysis (STR)
PCR analysis
Specialized Techniques
mitochondrial DNA analysis
Single Nucleotide Polymorphism (SNP)
Genetic fingerprinting/DNA profiling
DNA profiling refers to the process of analyzing DNA variations for the purpose of identification of individuals and determine familial relationships.
identification of crime suspects
Studying kinship
Sex identification
Archaeogenetics: Using DNA to study human prehistory
Paternity testing
identify victom and individuals that are unrecognizable due to natural disaster
missing children
Short Tandem Repeat analysis (STR)
molecular bio method used to compare allele repeats at specific loci in DNA between two or more samples
STR is microsatellite DNA with repeat units that are 2 to 7 base pairs in length
the number of repeats varying among individuals
evidence from crime scene
biological sample collected like blood, saliva, semen or muscle tissue etc.
DNA isolation
PCR amplification
Gel electrophoresis
STR profile genration
Alignment with SRT database
Purpose
paternity disputes
missing persons cases
DNA testing in forensic labs