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Animal Cell Culture, example, Definition, Definition, Advantages &…
Animal Cell Culture
Primary Explant Culture
Definition
A method to culture small pieces of tissue which has been removed surgically from animal tissue or organ (Rao, 2020).
Steps Overview
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- Cut and clean the explant
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- Subculturing and constructing cell line
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Cell Culture Systems
Adherent Cell Culture
Monolayer on artificial solid substrate, culture is growing on top of the layer (Thermofisher, n.d.)
Easy to implement for many cells, easier visual observations, but very limited in terms of growth due to limited surface area (Thermofisher, n.d.)
Suspension Cell Culture
Free floating culture in the culture medium (Thermofisher, n.d.)
Require cells that adapts to suspension culture, require regular cell counting, good for bulk or batch harvesting due to its larger volume for cell growth (Thermofisher, n.d.)
Primary Cell Culture
Samples grown and maintained from directly obtained/selected tissue explants OR from individualized cells obtained from the original/parental tissue
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Advantage: Best culture for In-vivo model/studies and they share the same karyotypes as their parent cells
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Cell line
When continuously subcultured, the primary culture can become an established cell line, which can be propagated repeatedly unlike the primary culture and has consistent properties.
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Continuous cell lines
A set of techniques used to reproducibly cultivate microorganisms at submaximal growth rates at different growth limitations in such a way that the culture conditions remain virtually constant (in ‘steady state’) over extended periods of time
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Disadvantages
The more aggressive the cell line, the more it changes over time in culture in cellular level
Not clear how the function of these cells relates to that of other cells (differentiation of healthy and diseased cells)
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