Criteria for inoculation development

Healthy

Stable

No contamination

Sufficient quantity

optimum size of each individual

Choice of microorganism for innoculum

High production

Nutrient: Cheap medium

Easy for genetic alteration

Easy optimal environment conditions

Easy to handle (suitable)

Fresh slant for 48 Hours

Roller Tube for 14-18 hrs at 25 RPM

Seed Sake flask 6-8 hrs at 300 RPM (28-30C)

Fermentation

Flat bed bottole

Shaker flask

Rotatory shker bed

small fermentor

large fermentor

Fist seed culture 2 dm3 flask 0.6 medium 48 hrs

2nd Stage seed 40-80 dm3 25-50 dm3 media 25-30 hrs

Production culture 500 dm3 and 300 dm3 medium140-160 hrs

1.5

Development of mycelium inocula Sporulation on

Solidified media eg clavulanic acid by streptomyces clevuligerus in Roux Bottle (Synthetic solids)

Solid media eg. Asparginase (anticance drug) from Streptomycese Rochei + natamycine (eye drop) from glyvosporus z28 ( Rice, barly, wheat and maiz) (Natural solids)

Submurged culture eg cheeze from penicillin notatum in 2.5% chloride soln