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Criteria for inoculation development, Choice of microorganism for…
Criteria for inoculation development
Healthy
Stable
No contamination
Sufficient quantity
optimum size of each individual
Choice of microorganism for innoculum
High production
Nutrient: Cheap medium
Easy for genetic alteration
Easy optimal environment conditions
Easy to handle (suitable)
Development of mycelium inocula Sporulation on
Solidified media eg clavulanic acid by streptomyces clevuligerus in Roux Bottle (Synthetic solids)
Solid media eg. Asparginase (anticance drug) from Streptomycese Rochei + natamycine (eye drop) from glyvosporus z28 ( Rice, barly, wheat and maiz) (Natural solids)
Submurged culture eg cheeze from penicillin notatum in 2.5% chloride soln
Fresh slant for 48 Hours
Roller Tube for 14-18 hrs at 25 RPM
Seed Sake flask 6-8 hrs at 300 RPM (28-30C)
Fermentation
Flat bed bottole
Shaker flask
Rotatory shker bed
small fermentor
large fermentor
Fist seed culture 2 dm3 flask 0.6 medium 48 hrs
2nd Stage seed 40-80 dm3 25-50 dm3 media 25-30 hrs
Production culture 500 dm3 and 300 dm3 medium140-160 hrs
1.5