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Nucleic Acid Isolation - Coggle Diagram

- - - - Na precipitation by Spin Columns
  - - - Remove RNA w/ Rnase enzyme
      - Protein removed by shaking solution in reagent that will denature proteins not nucleic acids (phenol)
        
        Emulsion centrifuged to remove denatured protein (DNA in aqueous solution is recovered)
  - - - After centrifugation DNA pellet is redissolved in buffer containing EDTA (usually TE bugger
      - Can be stored at 4C for at least a month and DNA sol can be frozen but avoid repeated freezing and thawing
- - - - e.g. 50A 260dilution factor = conc of DNA sample (ug/mL)
      - Most NAs absorb highly @ 260nm
    - - Approx 1.8 'pure' DNA
      - Approx 2.0 'pure' RNA
      - <1.8 indicates protein contamination (abs @ 280 nm)