Please enable JavaScript.
Coggle requires JavaScript to display documents.
Biotechnology, Transcriptors - Coggle Diagram
Biotechnology
Moderns biotechnology
Analyzing DNA molecules
By agarose gel electrophoresis
The mixture of DNA fragment is placed inside small wells
Ethidium Bromide (EtBr)
DNA ladder
DNA sequencing techniques
Frederick Sanger invented: chain termination method
Requires a thermostable DNA polymerase.
Taq polymerase is the most common
Determine the sequence of the DNA template
"Massive parallel" system
Terminator-dye nucleotides
Gene transfer in plants
Agrobacterium tumefaciens
Crown gall disease
Transfer DNA/T-DNA
A. tumefaciens
Ti plasmid
Bacterial origin of replication and selectable antibiotic resistance marker genes
Traditionals biotechnology
Fermentation
Domestication
Transcriptors
Is the study of trascriptomes
Studied by microarray analysis
After reverse trascriptase
Labelled cDNAs
Cancer research