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Diagnostic Reagent Strips as Biosensors - Coggle Diagram
Diagnostic Reagent
Strips as Biosensors
Biosensor
device that uses
biological material
in the detection of
substance of interest
Enzymes are v
effective
biosensors
specific - used to identify
individual molecules
quantitative - can tell how
much of a substance there is
molecule being monitored reacts
with immobilised enzymes + reaction
causes...
colour change
converted into electrical signal
Glucose
Monitoring
Clinistix
used to test presence of
glucose in urine
mechanism of action involves
enzyme glucose oxidase
METHOD
1-strip dipped urine
2-if glucose present - glucose oxidase
catalyses reaction - produces hydrogen
peroxide
3-second enzyme - peroxidase (in reagent
strip) uses hydrogen peroxide to change
colour of strip
4-colour change compared reference
chart to estimate conc
monitor
level
of glucose
glucose oxidase in another
biosensor = blood glucose meter
blood (finger prick) added to
disposable test strip w electronic
blood glucose meter
reaction catalysed by glucose
oxidase results electrical current
produced + digital reading
Pregnancy
Tests
antibodies in test strips
react with specific proteins
(hormones/atigens) to give +ve test
1-as result of pregnancy, levels
reproductive hormones (hCG)
increase as released from placenta
after implantation
2-hormones combine w
antibody-enzyme complex
in test strip
3-enzyme activated
to produce characteristic
colour change
antibody in test strip
detects presence
of hCG (human
chorionic
gonadotrophin)