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Immobilised
Enzymes - Coggle Diagram
Immobilised
Enzymes
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Definition
trapped within, or attached to,
appropriate insoluble inorganic
or organic materials
enzymes can be physically, or chemically
secured on or inside insoluble support
materials like fibres/gels/plastic beads
4 main
methods
Adsorption
D: enzymes attached, by weak forces
to inert substance (glass/a matrix)
Adv - relatively straight forward,
inexpensive + substrate/product not diffuse
through gel barrier (like entrapment)
Disadv - not always reliable (enzymes
easily washed from adsorptive material) +
some active sites inaccessible (part
of enzyme attached to glass)
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Advantages
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no contamination of end
product - simplifies downstreaming,
reduces purification costs + avoids
possible allergic reactions in consumers
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Continuous Flow
Processes
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- rates of flow
- methods of immobilisation
- environmental conditions
can be controlled to maximise
efficiency