proteins can be denatured at certain temperatures leading to change of shape of enzymes, can no longer bind with substrate. All reactions are faster at a higher temperature. However, enzyme-catalyzed reactions become slower or stop if the temperature becomes too high. The optimum temperature is usually around body temperature (37°C).

higher concentration of substrate increases the rate of reaction as the available enzymes would be working at their maximum rate

speeds up reactions until substrate amount Is limited

higher or lower in acid or alkaline can interfere enzyme structure

more heat = more kinetic energy (however see 'temperature')

competes for active site so the substrate could not bind with the enzyme

alters the structure of enzyme as it binds to the other parts of the enzyme so the active site is no longer functional

transfers chemical group from one molecule to another

catalyze the joining of two large molecules by forming a new chemical bond

catalyze the breakdown of chemical bonds through methods other than hydrolysis or oxidation

catalyze bond cleavages by reaction with water resulting in the division of a larger molecule into smaller molecules

catalyze electron transfer from one molecule to another

catalyze reactions involving a structural rearrangement of a molecule

present in the metabolism and genome of most living organisms

changes salt concentration, adds or removes cations & anions, disrupts bonds, disrupts 3D shape, denatures protein

protease

lipase, protease, pancreatic amylase

37ºC Is our body's temperature therefore the different temperatures range from here. Collecting data from 5 samples, the temperature of 0.5% hydrogen peroxide would be 10ºC, 30ºC, 50ºC, 70ºC, and 90ºC. Water should not boil for safety reasons.