Please enable JavaScript.
Coggle requires JavaScript to display documents.
Connections between the Genetics Unit and the DNA Extraction Lab - Coggle…
Connections between the Genetics Unit and the DNA Extraction Lab
Specimen
Name
Banana
Phenotype
Botanically identified as a berry
Traits
An elongated, curved fruit
It has an often yellow peel
The color on the surface of the fruit, just below the peel, is often a shade of white
The color deep into the fruit is often a shade of yellow
Some alleles of a banana's genotype have seeds in them, and others do not. This is often caused by mutation or modification in the organism's genome. The one used in the experiment had no seeds.
Experiment Goal
Extraction of DNA from a specimen
Process
Step 1: Cut up the specimen into small pieces
Step 2: Place the specimen into a plastic bag and mash it with your hands until it's mostly liquid
Step 3: In a separate cup, mix 2 teaspoons of dish soap, one teaspoon of salt, and around 120mL of water.
Step 4: Add 2 teaspoons of the extraction liquid into the bag, and massage it for around a minute
Step 5: Filter the solution through an old cloth or coffee filter and into a glass. The specimen's pulp should remain in the filter, while liquid should build up in the glass. Then wait for 2 minutes
Step 6: Pour ice cold ethanol down the side of the glass, and wait for around a minute. The ethanol should have precipitated the DNA out of the solution
Step 7: Use a toothpick or a fork to take the DNA out of the solution
Showcases the result of the experiment
Separates the liquid from the pure DNA
Separates the cell walls from the DNA-rich liquid we need
Made sure the extraction liquid reached all cells
The dish soap breaks down the cells' membranes, releasing the DNA
The salt breaks down the protein chains, keeping the chromosomes from the cells' nucleuses packed
Increases surface area even further, while breaking down some cell walls
Increases Surface Area of Specimen