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Light controllable platform for studying fast mechanical signals in cells …
Light controllable platform for studying fast mechanical signals in cells
Calcium signals
Detector: fluorescent dye JRCaMP1b
Secondary signal molecule in cellular mechanosensing
MDCK cell line
epithelial cells
Stimulation
No response to DR1 glass height
Response to flow
Calsium signal spreads to neibouring cells
Through cap junctions
Verified using cap junction blockers and openers
Material
DR1-glass
Topography changes when exposed to laser
Can be simulated using lasers in confocal microscopy
Higher laser intensity affects:
Topography (increase)
Flow rate (increase)
Glass flows when exposed to laser