4.2 Enzymes
Explain the role of enzymes in catalysing reactions that affect metabolism at a cellular and whole organism level.
click to edit
Explain the role of enzymes in catalysing both intracellular and extracellular reactions, to include catalase - intracellular reactions and amylase and trypsin extracellular reaction.
Describe and explain the mechanism of enzyme action, include lock and key hypothesis, induced-fit hypothesis and lowering of activation energy.
Describe the effects of:
pH
Temperature
Enzyme concentration
Substrate concentration
Explain the use of and calculate the Q10 temperature coeffficient
Describe practical investigations into the effects of pH, temperature, enzyme concentration and substrate concentration on enzyme activity. (include graphs of typical results, common limitations etc)
Explain the need for coenzymes, cofactors and prosthetic groups in some enzyme-controlled reactions, to amylase, carbonic anhydrase and
the role of vitamins
Explain ) the effects of inhibitors on the rate of enzyme controlled reactions, to include competitive and non-competitive and reversible and non-reversible inhibitors.