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Molecular basis of Inheritance - Coggle Diagram
Molecular basis of Inheritance
Identifying DNA as genetic material
Avery, McLeod, McCarty experiment
Concluded that transforming agent is DNA
Heat killed S cells, heat killed R cells, living S cells, living R cells
Hershey and Chase
Bacteriophage
Protein coat - radioactive sulfur (35S), DNA - radioactive phosphorus (32P)
Concluded that DNA is genetic material of virus that is needed
Griffith experiment
Concluded that transforming agent transforms/changes R cells into S cells
Mice and bacteria - streptococcus as pneumonia (2 strains)
Identifying DNA structure
Rosalind Franklin
DNA molecules have sugar phosphate backbone on outside
X ray diffraction image of DNA
Identified helix with 2 strands
Edward Chargaff
Base composition between species varied
A = T and C = G
Watson and Crick
Antiparallel strands
Complementary base pairs
DNA is a double helix
Purine - A and G, 2 rings
Pyrimidines - C, U, and T, 1 ring
DNA replication and repair
Replication
Unwinds bidirectionally
New DNA made from 5’ to 3’
Leading strand
continuous replication
Lagging strand
discontinuous replication
Okasaki fragment
Replication forks, replication bubble
Components
Helices
SSBPs
Primase
Primer
DNA polymerase I
Topoisomerase
Ligase
DNA polymerase III
Proofreading and repair
DNA polymerase
Mismatch repair enzymes
Recognize, remove, repair
Telomeres
Semiconservative model
New DNA molecule contains 1 old/parent strand and 1 new strand
Messelson and Stahl
Labeled nitrogen
Incorrect models
Conservative model
Dispersive model