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Antibiotic Sensitivity Test - Coggle Diagram

- - - - Need to prepare a bacterial suspension from the pure
        culture
        
        Pick a sterile test tube and mark the number & add 1-2 ml of the molar hidden growth
        
        sterilize the wire loop and keep it for cooling
        
        pick the bacterial colonies from the pure culture & transfer to the test tube containing growth to prepare a bacterial suspension
        
        Convert the bacteria with 0.5 maxwell and standards
        
        Label the mola hindu naga with test culture details or with the identification numbers
        
        Take the sterile cotton swab and the bacterial suspension dip the swab into the bacterial growth
        
        Take it out and remove any excess liquid by pressing the swab against the side wall of the tube
        
        open the plate and inoculate as long culture
        
        wipe the swap inside two side motion, gently the idea is to create
        uniform layer of bacteria
        
        after completely swabbing the plate in one direction slightly rotate the plate and continue swabbing and repeat the swapping process
        
        run the swap around the circumference of the plate close the plate and then dispose the swab in a biohazard container
        
        place antibiotic disc onto the swag dagger surface
      - first select the antibiotics you can transfer the disc with the help of a forceps or also you can use a multi disc dispenser
        
        now sterilize the tip of the forceps and pick up the disc carefully from one antibiotic set and place the disc onto the swab dagger surface
        
        keep at least 25 millimeter distance from this to disk maximum 6 discolor should be applied on a 90 mm plate
        
        close the platelet and keep the plate in an incubator at 37 degrees celsius in inverted position for overnight
  - - - in this method the test organism is inoculated on one part and the control organism in the other part of the plate with a little gap in the middle
        
        make sure the uninoculated gaps leaving a distance of three to four millimeter wide is needed when performing the dust
        
        once the inoculation is finished place the antimicrobial disc in the gap between the test and control strain by using sterile forceps
        
        then incubate the plate aerobically at 37 degrees celsius for 18 to 24 hours (incubation measure the radius of the inhibition sawn)
        
        Check the zone in test & control area from the middle of the disc to the edge
- - - - 4) Start by flaming the top of the tube
        
        5) Remove a sterile cotton swab from the package and dip it into the test tube and then press the cotton swab against the inside of the tube to remove excess broth
        
        6) Take the cotton swab with bacteria and cover the entire surface
        
        7) Move the cotton swab back and forth covering as much of the auger as possible
        
        8) Rotate the plate and swab and repeat the process.
        
        9) Next is for the filter paper disc where it is filled with chemicals
        
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