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Qaisara WA3 bio revision - Coggle Diagram
Qaisara WA3 bio revision
Nutrient groups
Test for Carbohydrates
Iodine test for presence of starch
Positive result: Blue-black
Negative result: No change in colour
Benedict's test for the presence of
reducing sugar
Eg. of reducing sugars: maltose, lactose
Positive:
Brick-red
ppt will form
Conclusion:
Large
amount of RS is present.
Negative: Remains blue, no change in colour
Positive: Green ppt
Conclusion:
Low
amount of RS present
Test for Proteins
BIURET TEST
(Biuret reagent is 0.75 mM copper (II) sulfate and 94 mM sodium hydroxide.
POSITIVE RESULTS
VIOLET COLOURATION IS OBSERVED .
(Nitrogen atoms form complex with Cu2+ ions)
NEGATIVE RESULTS
REMAINS BLUE.
Test for Lipids
ETHANOL-EMULSION TEST
POSITIVE RESULT
CLOUDY WHITE EMULSION IS FORMED (like like like got cloud)
NEGATIVE
Solution remains clear
Carbohydrates
____saccharides
Monosaccharides
Glucose
Galactose
Fructose
Di
saccharides
Glucose + fructose = sucrose
Glucose + glucose = maltose
Glucose + galatose = Lactose
Proteins
Fat
Digestion
Mouth
Salivary amylase (pH ~7) digests
starch to maltose
Stomach (pH ~2 from HCl)
Chemical (gastric juice)
Pepsin breaks down proteins to form polypeptides
Mechanical
Churning (contraction of stomach muscles) of food pieces to increase area
Small intestine
Pancreatic juice (pH ~8)
Trypsin breaks down protein to form polypeptides
Pancreatic amylase breaks down starch to maltose
Pancreatic lipase breaks down small droplets to form fatty acids and glycerol
Intestinal juice (pH ~8)
Intestinal lipase breaks down small fat droplets to form fatty acids and glycerol
Intestinal amylase breaks down
maltose
to glucose
Peptidase
breaks down polypeptides to form amino acids.
Pancreas
Produces pancreatic juice
Liver
Produces bile
Emulsifies fat into
small fat droplets
Gall bladder
Stores bile
Enzymes
Keywords: Catalyst, speed up reaction of enzyme, Lock-and-key, hypothesis, substrate bind to enzyme, activation energy, pH level, rate of enzyme activity
4 stages of Lock-and-key
Substrate entering active site of enzyme (E+S)
ENZYMES/SUBSTRATE COMPLEX (ES)
3.ENZYME/PRODUCT COMPLEX (EP)
4.PRODCT!!!!!!! (E+P)