LAB 4
Morphological description for the identification of Hypopygiopsis tumarasvini Kurahasni (Diptera: Calliphoridae )
Key for identification of third instars of European Blowflies (Diptera: Calliphoridae) of Forensic Importance
Each anterior spiracle had eight to eleven papillae organised in a single row. Between the prothorax and the mesothorax, the dorsal spines were organised in groups of posteriorly projecting acuminate spines with darkly pigmented ends. Each of the posterior spiracles has three distinct and separated long, slender spiracular slits that are ringed by a black, thick peritreme that wraps around a button ventromedially. Between the middle and lower spiracular slits, there is a prominent inner extension of peritreme.
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Observation on morphology of immature Lucilia porphyrina (Diptera: Calliphoridae), a fly species of forensic importance
Result
Discussion
Materials and methods
Conclusion
Introduction
The case study on third instar of Calliphoride for forensic investigationin Europe.
European larvae of blowfly are involved in exposed vertebrate includinf human.
*11 species widespread through Europe from both real cases and carrion experiments
Blow flies of the genera Lucilia are well known to forensic entomologists as their immature stages are found to related with human corpses. The major aim of this study was to present the important characteristics of L. poryphyrina larvae for potential use in identification.
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The first instar body length 2.64 +- 0.24 mm (n=21). The fist instar is smooth and has 12 segments, with the last abdominal segment being truncated.
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The third instar of L. porphyrina is morphologically different from previous descriptions of L. cuprina third instar, which can be distinguished by the difference in
-size of the posterior spiracle
-prominent inner projection between the middle and lower slits
-degree of sclerotized peritreme
- the number of papillae on the anterior spiracle
The larvae and adults of Hypopygiopsis tumrasvini Kurahasi, Diptera, Calliphoridae, a blow fly species that maybe of forensic interest, are described in this article.
The cepha-lopharyngel skeleton, anterior and posterior spiracles, and dorsal spines between the prothorax and mesothorax are all illustrated in second and third instar larvae, with an emphasis on important characteristic features used for larval identification, such as the cepha-lopharyngeal skeleton, anterior and posterior spiracles, and dorsal spines between the prothorax and mesothorax
Fly colony
Light microscopy observation
Morphometric Measurement
Scanning electron microscopy electron
The second instar body length 4.21 +- 0.53mm (n=10). The second instar is 1.6x as long as the previous one and its head is more tapered.
The third instar body length robust, lengthening to 12.04 +- 1.77mm (n= 36). The head segment is much smaller than the thoracic and abdominal segments.
Puparia length, 7.13+- 0.49 mm, width 3.11+- 0.22mm (n=21) and tapered anteriorly.
Calliphora vicina
C. vomi toria
Chrysomya albiceps
Phormia regina
Protophormia terraenovae
Lucilia caesar
L. illustris
L. sericata.
3 additional species
Cynomya mortuorum (human corpses at least twice)
Chrysomya megacephala
Lucilia ampullacea.
Third instars of C. vicina, C. vomitoria, P. regina, P. terraenovae, L. ampullacea, L. illustris, and L. sericata were bred from eggs deposited by females collected in the city of Toruń in Northern Poland.
Third instars of C. mortuorum, Ch. albiceps, and L. caesar were collected during research on the insect succession on pig carrion conducted on Biedrusko Military Range in Western Poland.
(SDF) may vary according to the size of the maggots and the techniques of preparation.
measure only for freshly killed larvae (fully grown)
Procedure
All larvae were killed by soaking in hot water (about 95°C)
Then stored in 80% ethanol.
For slide preparation
Larvae were macerated for 24 h in a cold solution of 5% KOH.
Particular fragments of the body were mounted in Hoyer’s medium or dehydrated through 80%, 90%, and 99.5% ethanol and mounted in Euparal.
The spiracular distance factor was calculated according to SDF = a/b.
Discussion
Conclusion
References
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