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LAB 4 - Coggle Diagram

- - - - Procedure
        
        All larvae were killed by soaking in hot water (about 95°C)
        
        Then stored in 80% ethanol.
        
        For slide preparation
        
        Larvae were macerated for 24 h in a cold solution of 5% KOH.
        
        Particular fragments of the body were mounted in Hoyer’s medium or dehydrated through 80%, 90%, and 99.5% ethanol and mounted in Euparal.
        
        The spiracular distance factor was calculated according to SDF = a/b.
- - - - Fly colony