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Biochemical Engineering - Coggle Diagram
Biochemical Engineering
Substrate Preparation
Elemental composition - water, carbon, nitrogen, micro/macro-elements
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Cellular control of enzymatic pathway - enzyme production/ compartmentalization/ inhibitor & activators/ post-translational modification/ respond to environmental changes
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Microbial Growth
Growth phase: lag, exponential, stationary, death
Growth yield: ratio of biomass produced to substrate consumed (limiting-substrate control the yield)
Product yield: ratio of product synthesized to substrate consumed (variable)
-> Generation time/ doubling of cells - specific growth rate (specific to strain and used of media)
Product synthesis: Leudeking-Piret type eq.
Monod model - describe the substrate-limited growth
Cell growth inhibition: byproduct, environment (temperature/pH), substrate
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Scale-up
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Factors were considered after the successful lab-scale experiments: fermentation/ product recovery & purification/ effluent treatment
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