Please enable JavaScript.
Coggle requires JavaScript to display documents.
spectrophotometer - Coggle Diagram
spectrophotometer
-
what
quantitave measurement, absorbance of chemical substances, passing a beam of light, specthrophotometer
Light
-
wave and particles
-
travels in a speed of lights, in vacuum
v=c/λ , c = 2.998 x 108 ms-1
Quantum mechanics
photon; As a particle, it interacts with matter by transferring its energy E: E = hc λ-1 , h = Planck’s constant, 6.63 x 10-34 Joules
principle
-
-
Beer Lambert Law
-
Quantitative measurement, A = Elc
The importances
Allow determination of about 20 elements in biological samples- (calcium, magnesium and manganese)
Absorption spectrophotometer is more sensitive than emission instrument and can detect 1ppm each of the common element with exception of alkali metals
-
-