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Subheading of hgh - Coggle Diagram
Subheading of hgh
6: transformation
E. Coli as a host celll
Recombinant vector transform in host cell E.Coli
E. Coli produce hgh
E. coli isolate from culture
Isolate hgh by fermentation technology
Application of hgh
)
3;reverse transcriptase:
cDNA fromed from mRNA
cDNA obtained by reverse transcription
CDNA is gene for for hgh
Gene cut with endonuclease enzyme to remove 24 amino acid
5:Selection of suitable vector
**PBR322 used as a vector :
Hgh gene ligate in to restricted sites of vector
Just downstream of lac operator region of vector
2: isolation of mRNA
MRNA isolate from pituitary gland
4: joining of synthetic gene and cDNA
T4 enzyme ligase use to join gene and CDNA
:
1:chemical synthesis of gene for first 24 amino acid
Steps
1:Chemical synthesis of gene for 24 amino acids
2:isolation of m RNA for hgh
3:Reverse transcription:
4: selection of suitable vector and recombination
5: selection and recombination in suitable host cell
Gene construct in fragments
Join by ligase enzyme
Chemical synthesis 24 Amino acid
