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Isolation of Microorganism - Coggle Diagram
Isolation of Microorganism
Microorganisms
speed of growth
can undrgo genetic manipulation
ease of cultivation
use cheap substrate
diversity of potential product
Screening & Isolation
Sources
Culture collection
Natural environment (Bioprospecting)
Important properties of industrial microorganisms
simple nutrional requirement
optimum temperature 40 dc
easy to maintain and grow
The productivity
The stability of the organism and its amenability to genetic manipulation
The ease of product recovery from the culture
Non-pathogenic, GRAS
Petri dish
shake flask
Isolation & sample collection
isolation methods
dilution
gradient plate
sponging
flotation
differential centrifugation
enrichment in liquid culture (flask)
isolation method
Enrichment in liquid medium
Screening based on the desired
characteristics
Solid media
selective media
selective vs differential