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Gel electrophoresis - Coggle Diagram
Gel electrophoresis
General principle
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anode & cathode electrodes, electrolyte
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Anode - (+ve), Cathode - (-ve)
Electric potential generated, e move from anode to cathode
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General operation
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Power supply
Simple power supply can be regulate by voltage, max 200V
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IEF
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Principle
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linear relationship between the logarithm of the molecular weight
and the relative distance of migration of the SDS-polypeptide complex.
Why DNA (-ve)ly charged
Presence of phosphate gp/ phosphate backbone is (-ve)ly charged/ due to the presence of bonds created between the phosphorus and oxygen atoms.
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