NCBI for all of the major databases, including PubMed, Nucleotide and Protein Sequences, Protein Structures, Complete Genomes.
Literature databases:
— PubMed, PubMed Central, Books, OMIM
— Molecular databases:
• Sequences:
EST, STS, GSS, HTC, FLIC, UniGene, RefSeq, HomoloGene
• Structures:
MMDB, CDD,
• Taxonomy
— Other databases:
GEO, SKY/CGH
pubMed
PubMed database was designed to provide access to citations (with abstracts) from biomedical journals.
Subsequently 🡪 A linking feature was added to provide access to full-text journal articles as well as other related web resources
PubMed Central (PMC)
It is the National Library of Medicine's digital archive of FREE full-text journal literature.
Certain journals allow the full text of their articles to be viewed directly in PMC.
Other journals require that PMC direct users to the journal’s own website to see the full text of an article.
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Nucleotide database
Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery.
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Gene
Gene database is a free online resource for gene sequences from many species and organisms.
The NCBI gene database includes gene sequences, gene alleles and mutations, genomes, pathways, protein sequences and so much more.
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Protein databases have become a crucial part of modern biology. Huge amounts of data for protein structures, functions, and particularly sequences are being generated. These data cannot be handled without using computer databases.
Searching databases is often the first step in the study of a new protein.
Comparison between proteins or between protein families provides information about the relationship between proteins within a genome or across different species, and hence offers much more information than can be obtained by studying only an isolated protein
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Blast Basic Local Alignment Search Tool , which is an algorithm based search tool used for comparing and finding regions of local similarity between sequences
BLAST tool compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches.
More or less like running a “correlation” algorithm
BLAST program used a protein / nucleotides “query” sequence to scan a protein / nucleotides sequence database
Blast P
the accession number 204309 of protein and useing the accession number of FASTA format of protein and search on plast p
Blast X
BLASTX with accession number of FASTA format of gene 15112000
the final result is is greater than allowed on the BLAST
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tblastn
the accession number 204309 of protein and useing the accession number of FASTA format of protein in tblastn
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