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CHROMATOGRAPHY, Factors effect, phase, polar, non polar, polar, non polar,…
CHROMATOGRAPHY
Affinity
Spacer arm
Ligand (adsorbent)
Matrix
Separated involved biorecogniton element
Selectively
Strong interaction between the sample and stationary phase
Isolation & Purification
Elution method
Ionic strength
Competitive
Reduced polarity
pH elution
Chaotropic
Gas
mobile phase
Column (resin inside)
Indicator to identify components that needed to observe
Nitrogen
Short retention time means gas does not stay longer = the movement does not restricted
Mass can be determined
Physical Characterization
Water Regain and Bed Volume
Gel Particle Shape and Size
Fractionation range
Void Volume
Exclusion limits
Elution Volume
Planar
Gravity
Capillary
Partition Principle
liquid-liquid
Reverse
Stationary
mobile
Normal
Mobile phase
hexane
Stationary phase
alkylamine
Size
Gel Fitration
Ion exchange
anion & cation
Involved electrostatic interaction
Nature of solute molecule
Selection based on the charged group
Separation of components in a complex mixture
Identification of components
Purification of the components
Verification of components
Quantification of the components
Techniques
Adsorption
ion exchange & Column
Molecular exclusion
Partition
Planar & Gas
Affinity
Types of Gel
Agarose
Polyacrylamide
Dextran
Combination of polyacrylamide-dextran
Sample (solute) is allow to interact with 2 physically distinct entities;
Stationary phase
Mobile phase
Factors effect
phase
polar
non polar
polar
non polar
Selection of ion exchange
Retention time
carrier gas
components
characteristics
Type
Liquid-gas partitioning
Liquid-liquid partitioning