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🩸 HEMOCYTOMETER 🧬, Groups Member 👫🏻 - Coggle Diagram
🩸 HEMOCYTOMETER 🧬
Sample Preparation 🧫
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Reagents ⚗️
Turks Solution
1% Formalin in 3% Sodium Citrate Solution
Procedure 🧪
- Warm the culture medium to 37˚C
- After preparing a cell pellet (refer to Preparing a Cell Pellet), resuspend it in 1-5 mL of culture medium, making note of the media volume added
- Pipette 10 uL of cell suspension and place into one hemocytometer chamber
- Carefully place a coverslip on top, ensuring that no bubbles are trapped in the system
- Place the hemocytometer under a microscope
- Using the cell counter, count the cells in the squares outlined below. Make sure to NOT count cells that are sitting on dotted lines. If used trypan blue, make sure to differentiate counting for live (normal) and dead (blue) cells
Cell Calculation 🧮
Calculation of RBC Count
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Calculation of WBC Count
One large area is 1 x 1 mm, and the depth is 0.1 mm.
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