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HEMOCYTOMETER, WhatsApp Image 2021-05-02 at 13.31.51 - Coggle Diagram

- - - - 6) The solution present mixed and 2 drops of the solution discarded from pipette
      - 7) The tip of the pipette touched with the hanging drop against the edge of 45° approximately
      - 5) The Hemocytometer and coverslip wiped to clean the dust using swab
      - 8) Small amount of the solution fill into the chamber.
      - 4)The RBC pipette rotated to mix the blood and Hayem's fluid
      - 9) After 3 to 5 minutes when the cells settled down, the chamber placed on the stage of microscope to count the RBC
      - 3) The RBC pipette filled up with Hayem's fluid up to 101 mark
      - 2) The outside of the pipette wiped with cotton to prevent false high results
      - 1) The RBC pipette filled up to 0.5 marks with blood
    - - 4) The Hemocytometer and coverslip wiped to clean the dust using swab
      - 5) The diluted solution mixed and discarded 2 drops before the chamber charged
      - 3) The solution mixed well
      - 6) the rubber tube of RBC pipette gently pressed to drop the fluid in hanging position
      - 2) 0.02ml of blood specimen filled in the test tube using RBC pipette
      - 7)The tip of the pipette touched with the hanging drop against the edge of 45° approximately
      - 1) 3.98ml of the Hayem's fluid filled in test tube
      - 8) Small amount of the solution into the chamber
- - - - Procedure is same as the RBC methods. However, different dilution factor
      - 1) The WBC pipette filled up to 0.5 marks with blood.
      - 2) The outside of the pipette wiped with cotton to prevent false high results.
      - 3) The WBC pipette filled up with Turk's fluid up to 11 mark.
      - 4)The WBC pipette rotated to mix the blood and Turk's fluid.
      - 6) The solution present mixed and 2 drops of the solution discarded from pipette.
      - 8) Small amount of the solution fill into the chamber.
      - 5) The Hemocytometer and coverslip wiped to clean the dust using swab.
      - 7) The tip of the pipette touched with the hanging drop against the edge of 45° approximately.
      - 9) After 3 to 5 minutes when the cells settled down, the chamber placed on the stage of microscope to count the WBC.
    - - 4) The Hemocytometer and coverslip wiped to clean the dust using swab.
      - 5) The diluted solution mixed and discarded 2 drops before the chamber charged.
      - 3) The solution mixed well.
      - 6) The rubber tube of WBC pipette gently pressed to drop the fluid in hanging position.
      - 2) 0.02ml of blood specimen filled in the test tube using WBC pipette.
      - 7)The tip of the pipette touched with the hanging drop against the edge of 45° approximately.
      - 1) 3.98ml of the Turk's fluid filled in test tube.
      - 8) Small amount of the solution into the chamber.