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TILLING, Induced Mutation - Coggle Diagram
TILLING
Mutation calling methods
Pooling
Methods
Bi-dimensional Pooling
Poisson Distribution
High-throughput sequencing
DNA Library
ComSeq
CAMBa
Creation of Mutated Population
M2 Population
DNA Isolation
Detection of mutations
Analysis of the mutant phenotype
M3 Seed Bank
TILLING Platform
Forward-Genetic
Induced Mutation
Targeted Mutagenesis
Experiment Location
In-vitro
Programmable nucleases
CRISPR
Reverse Genetic
ZFNs
TALENs
Error-Prone PCR
Random Mutation
PFunkel
MAGE
In-vivo
Orthogonal DNA polymerase-plasmid pairs
TaGTEAM
Random Mutation
Forward Genetic
Retrotransposon-based targeted mutagenesis
Affected Lesion/s
Site-Targeted Mutagenesis
Multi-Site Mutagenesis
Mutation
Strategy
Forward-Genetics
Genetic Information
Reverse-Genetics