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Leah Neimeister and Destiny Anderson Non-fermenting Gram-Negative…
Leah Neimeister and Destiny Anderson
Non-fermenting Gram-Negative Bacilli
Stenotrophomonas maltophilia
Gram Stain
pink rods
long and thin
clusters
Colony Morphology
Sheep Blood Agar:
gamma-hemolysis
round, raised, smooth, shiny/mucoid
MacConkey Agar:
non-lactose fermenter
yellow agar, translucent colonies, pinpoint, irregular margins
Pseudomonas Isolation Agar (cetrimide):
slight yellow, goopy mucoid colonies
DNAse Agar:
+
clearing of agar around streak
Biochemical Tests
Catalase:
-
no bubbles
(expected result: +)
Oxidase:
-
no color change
TSI/H2S:
K/K
red slant/red butt
Citrate:
-
no color change
Urease:
+
light pink slant/peach butt
O/F:
non-oxidizer
aerobic - green top and butt
non-fermenter
mineral oil - green top and butt
Motility:
+
can't see stab streak
Bile Esculin:
+
black agar
ONPG:
+
yellow/orange color change
Nitrate Reduction:
+
red color change after reagents A and B
Egg Yolk Agar:
+
pink agar
opaque, burnt orange color
Acinetobacter baumannii
Gram Stain
pink rods
pleomorphic, short
pairs and clusters
Colony Morphology
Sheep Blood Agar:
gamma-hemolysis
round to punctiform, shiny/mucoid, raised, smooth, medium gray color
MacConkey Agar:
non-lactose fermenter
yellow agar, translucent colonies, pinpoint
Pseudomonas Isolation Agar (cetrimide):
translucent agar
Biochemical Tests
Catalase:
+
bubbles
Oxidase:
-
no color change
TSI/H2S:
K/K
pink slant/red butt
Citrate:
+
royal blue color change
Urease:
-
yellow slant and butt
O/F:
oxidizer
aerobic - yellow top/slight green butt
non-fermenter
mineral oil - green top and butt
Motility:
-
can see stab streak
Bile Esculin:
-
yellow agar
ONPG:
-
no color change
Nitrate Reduction:
-
pink color change after adding zinc
Egg Yolk Agar:
+
pink agar
opaque, salmon color
DNAse Agar:
-
no clearing of agar around streak
Pseudomonas aeruginosa
Gram Stain
pink rods
long, thin, straight
clusters
Colony Morphology
Sheep Blood Agar:
beta-hemolysis
light gray opaque center with semi-clear border,
irregular margin, large, flat, scale-like, iridescent,
grape-like smell
MacConkey Agar:
non-lactose fermenter
yellow agar, translucent colonies,
flat, fuzzy margins with raised center
Pseudomonas Isolation Agar (cetrimide):
green agar and colonies
Biochemical Tests
Catalase:
+
bubbles
Oxidase:
+
purple color change
TSI/H2S:
K/K/H2S
pink slant/red butt/ black precipitate
(expected result: no H2S)
Citrate:
+
royal blue color change
Urease:
+
neon pink slant and butt
O/F:
oxidizer
aerobic - yellow top/green butt
non-fermenter
mineral oil - green top and butt
Motility:
+
can't see stab streak
Bile Esculin:
-
yellow agar
ONPG:
-
no color change
Nitrate Reduction:
+
no color change after adding zinc
Egg Yolk Agar:
+
pink agar
opaque, light burnt orange color
with some white spots inside
DNAse Agar:
-
no clearing of agar around streak
Pseudomonas putida
Gram Stain
pink rods
long, curved
pairs and clusters
Colony Morphology
Sheep Blood Agar:
gamma-hemolysis
large, hazy, fuzzy, faded, irregular margin,
light gray, opaque center with semi-clear border
MacConkey Agar:
non-lactose fermenter
yellow agar, translucent colonies, irregular, scaly
Pseudomonas Isolation Agar (cetrimide):
yellow agar
Egg Yolk Agar:
+
pink agar
opaque, peachy orange color
DNAse Agar:
-
no clearing of agar around streak
Biochemical Tests
Catalase:
+
bubbles
Oxidase:
+
purple color change
TSI/H2S:
K/K
pink slant/red butt
Citrate:
+
royal blue color change
Urease:
delayed +
light pink slant/yellow butt
O/F:
oxidizer
aerobic - yellow top/green butt
non-fermenter
mineral oil - green top and butt
Motility:
+
can't see stab streak
Bile Esculin:
-
yellow agar
ONPG:
-
no color change
Nitrate Reduction:
-
pink color change after adding zinc
Alcaligenes faecalis
Gram Stain
pink rods
medium size, chunky
pairs
Colony Morphology
Sheep Blood Agar:
gamma-hemolysis, green discoloration of agar
medium gray color, smooth, shiny/mucoid,
punctiform, raised, fuzzy margin
MacConkey Agar:
non-lactose fermenter
yellow agar, translucent colonies, filamentous
Pseudomonas Isolation Agar (cetrimide):
translucent agar, no growth or change
DNAse Agar:
-
no clearing of agar around streak
Biochemical Tests
Catalase:
+
bubbles
Oxidase:
+
purple color change
TSI/H2S:
K/K
pink slant/red butt
Citrate:
+
royal blue color change
Urease:
+
peach slant and butt
O/F:
non-oxidizer
aerobic - royal blue top/green butt
non-fermenter
mineral oil - green top and butt
Motility:
+
can't see stab streak
Bile Esculin:
-
yellow agar
ONPG:
-
no color change
Nitrate Reduction:
-
red color change after adding zinc
Egg Yolk Agar:
+
pink agar
opaque, peachy orange color