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Transplantation - Coggle Diagram
Transplantation
HLA system
Highly polymorphic
Evolved with us
e.g. in western Europe HLA-A2 positive is prominent (from time of plague)
HLA antigens found on short arm of chromosome 6 (6p21.30)
Inheritance
Codominant mendeliam inheritance
Inherited en bloc from parents in haplotypes
Nomenclature
Serologically defined
HLA-A2
39 alleles
Discovered using Ab's
Broad serology
Molecular
HLA-A*02:03
Testing
Lymphocytotoxic test (LCT) or Complement Dependant Cytotoxicity test (CDC)
In HLA typing plates
Small wells
Filled with mineral oil to prevent evaporation
Lymphocyte isolation
Whole blood sample diluted with tissue culture media laid across lymphoprep
Lymphoprep has same specific gravity as lymphocytes
Blood cells drop to bottom, platelets sit at top