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Breaking Seed Dormancy in Carex wahuensis - Coggle Diagram
Breaking Seed Dormancy in
Carex wahuensis
Collection
Air dried from 3 days up to 2 weeks.
In laboratory conditions
20-22C
40-60%RH
Removed from spikelet when seeds looked beige/brown
Sorted/Cleaned
unfilled achenes were removed
using an air column?
Perigynium removal (if not part of the experiment)
Placed in a paper bag
Seed Viability Testing
TZ testing
Germination testing
Collect and plant, no treatment?
Other...
Treatments
Bacon Slice
: removing a tiny part of the seed coat where the radicle would emerge
Exogenous GA3
: along a concentration gradient or with and without
Perigyinal Removal
: Presence and Absence potentially with another treatment
Stratification
Could place entire batches in 3 different storage regimes
Cold-Dry
Kept in refrigerator at 4C
Cold Wet
wrapped in 2 layers of filter paper, wet with distilled water and placed in sealed plastic bags at 4C
Dry-warm
kept in a laboratory drawer
15-25C
Post stratification
: Seeds will undergo different temperatures with light/dark conditions
Temperature regimes TBD
Altering photoperiod TBD
complete darkness
Remove x amount every month and count germination every 2 days.
Figure out monthly intervals of testing (i.e. every month for how long?)
Confirming PD: per DW email
Imbibe a seed set in darkness using aluminum foil
Put the seeds in the refrigerator then move to germination conditions.
Need to Figure Out
Total number of collected seeds
How long it takes to develop seeds?
spikes observed in march 2021
additional seed source!!
how many germination chambers will be available
at what temperature ranges?
Do I need to purchase additional items?