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chromatography, work by allowing the present in mixture to distribute b/w…
chromatography
history
1903 tswett-plant pigment separation
1938 TLC Ion exchange
1965 istrumental LC
component
Eluent
chromatography
Elutate
stationary phase
Purpose
preparative
principle
liquid phase
ion exchange chromatography
separation of charge biomolecule
clinical research
biotechnology
fermentation
analytic
ion Exchange chromatography
classification
technique by physical state of mobiel phase
liquid chromatography
gas chromatography
affinity chromatography
column chromatography
paper chromatography
TLC
tecnique on the basis of interaction to the stationary phase
ion exchange chromatography
adsorption chromatography
partition chromatography
column chromatography
Application
isolate active ingredient
separating compound mixture
Drug estimation from formulation
principle
stationary phase in tube
lower affinity to stationary phase
Advantage
disadvantage
complex mixture cannot separated
Less accurate compared
not effective in quantitative analyse
not occupy much space
unknown compound identify
Application
Ripening
to detect contaminated in blood
to detect aduterent
Rapid
cheaper
TYPE
Descending paper chromatography
Ascending paper chromatography
Thin layer chromatography
component
uses
to detect insecticide
use in forensics to analyse the dye composition of fiber
to detect pesticide
stationary phase
mobile phase
affinity
paper chromatography
principle
work by allowing the present in mixture to distribute b/w stationary and mobile phase
on the basis of chromatography bed shap
Application