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Vesicular Traffic, Secretion
and endocytosis, Secretory Pathway - Coggle…
Vesicular Traffic, Secretion
and endocytosis
Techniques for Studying
the Secretory Pathway
in live cells, you
need to label the proteins
pulse labeling with radioactive aa (can label a group of newly made proteins in ER)
- tracked by following compartment specific modification
Temperature sensitive mutants
will keep a group of misfiled newly made proteins in the ER
until the temperature allows them to fold and leave
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Secretory Pathway
Out of Cell (Secreted)
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Regulated
- stored in secretory vesicles
- wait neuronal or hormonal signal
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ER
folding+glycosylation
- anterograde transport = forward
- retrograde transport = backwards
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