MODEL ORGANISMS & CELL CULTURES for the DEVELOPMENT of NEW THERAPIES

CELL CULTURES

REGENERATIVE MEDICINE

HYBRIDOMA CELL LINE

VACCINE PRODUCTION

primary

secondary

growth rate

finite cell line

transformed cells

immortalized

chemically induced

virally induced

spontaneously

tumorigenicity assay

He-La cells

cancerous

grow unusually fast

immortal

bioethical issues

human mAb

B lymphocytes of the spleen
+
immortal myeloma cells

produced ex vivo

different from animal pAb

nerve regeneration

Stem Cell Therapy


burn care

cell culture-based influenza vaccines production

inoculation of virus

incubation

virus growth

separation, filtration, purification

testing and delivery

IMMUNOTHERAPY

human mAb

no rejection

difficult to generate


B lymphocytes of peripheral blood

mouse hybridoma manipulation

to detect unknown antigens

to discover cellular surface components

vectors to bring drug to the target cell

cancer cells