MODEL ORGANISMS & CELL CULTURES for the DEVELOPMENT of NEW THERAPIES
CELL CULTURES
REGENERATIVE MEDICINE
HYBRIDOMA CELL LINE
VACCINE PRODUCTION
primary
secondary
growth rate
finite cell line
transformed cells
immortalized
chemically induced
virally induced
spontaneously
tumorigenicity assay
He-La cells
cancerous
grow unusually fast
immortal
bioethical issues
human mAb
B lymphocytes of the spleen
+
immortal myeloma cells
produced ex vivo
different from animal pAb
nerve regeneration
Stem Cell Therapy
burn care
cell culture-based influenza vaccines production
inoculation of virus
incubation
virus growth
separation, filtration, purification
testing and delivery
IMMUNOTHERAPY
human mAb
no rejection
difficult to generate
B lymphocytes of peripheral blood
mouse hybridoma manipulation
to detect unknown antigens
to discover cellular surface components
vectors to bring drug to the target cell
cancer cells