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Question n. 53 Assess the usefulness and limitations of information…

- - - - Emission of secondary electrons
        from the surface of the specimen
        
        Negative staining
        
        Specimen +
        solution of heavy metal salt
        
        Reverse or negative image
        
        Possibility to observe
        macromolecular aggregates
        
        Cryoelectron microscopy
        
        Freezing of the specimen
        
        No need for staining
        
        Possibility to observe smaller structures
        
        Three-dimension image
        
        External surface of specimen
    - - Flow of electrons through the specimen
        
        Internal structure of specimen
        
        Two-dimension image
  - - - Localization of multiple proteins in a single sample
    - - Detection of antigen only
        on the surface of the specimen
  - - - Fluorescent species
        (fluorescein and rhodamine)
        
        Imaging structural components
        
        Viewing genetic material within a cell
  - - - Subjection to an electric field
        
        Migration of proteins on
        the basis of size and molecular weight
        
        Two-dimensional
        gel electrophoresis
        
        Two-dimension map of proteins
        
        Western blotting
        
        Identification of target protein through
        antibody-antigen interaction
- - - - FRET
        
        Distance in between two proteins
        
        Monitor the interaction of signaling molecules
        with their receptors
        
        See proteins in macromolecular complexes
        at specific locations
      - FRAP
        
        Movement of proteins inside the cell
        
        Analyze protein’s kinetic parameters
        
        Diffusion coefficient
        
        Active transport rate
        
        Binding and dissociation rates from other proteins
    - - High quality images
        
        Elimination/reduction of background information
        away from the focal plane
        
        Capability of collecting serial optical sections (time lapse)
        
        Ability to control depth of field