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Required Practical - Coggle Diagram
Required Practical
Method
- Sterilise the bench with ethanol and cotton wool
- Put a bunson burner on a heatproof mat and light it on a yellow flame.
- Mark the underneath of the petri dish by deviding the dish into 3 equal sections and label them 1, 2 and 3, put a dot in the middle of each section and write your initials, the date and the type of bacteria.
- Wash your hands with antibacterial handwash
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- Remove the lid of the bottle containing cultured bacteria then quickly flame the neck of the bottle with the bunson burner.
- Collect 1ml of the bacterial culture in a pipette.
- Quickly flame the neck of the bottle again.
- Carefully lift the petri dish at an angle and do not open it fully.
- Pipette the bacteria onto the petri dish and replace the lid. Place the pipette into the ‘discard beaker’. Turn the Bunsen burner flame back to yellow.
- Collect a bottle of agar from the water bath.
- Quickly lift the lid at an angle & pour the agar into the dish.
- GENTLY move it N-S-E-W to spread the agar evenly.
- Leave it to set for 5 minutes.
- Put different antiseptics onto the three filter paper discs. This can be done by either soaking them in the liquid or spreading the cream or paste onto them.
- Partially lift the lid of the agar plate and use sterilsed forceps to carefully put each disc onto one of the dots drawn on base of your Peri dish. Be careful not to touch the forceps onto the agar.
- Label which antiseptic is in each of the three numbered sections of the plate.
- Secure the lid of the agar plate in place using two small pieces of clear tape.
Do not seal the lid all the way around as this creates anaerobic conditions. Anaerobic conditions will prevent the E. coli bacteria from growing and can encourage some other very nasty bacteria to grow.
- Incubate the plate at 25 °C for 48 hours.
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